Alpha-Tubulin Housekeeping Cellular Kit
Total NFkB cellular kit HTRF®
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Ready-to-use
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High sensitivity
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Faster and more convenient than ELISA
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No-wash
Overview
Total NFkB kit serves as a normalization assay with our Phospho-NFkB kits. NFkB (Nuclear Factor Kappa B) is of great interest in cancer research and a key player in the inflammatory response. The buffers of both HTRF phospho- and total NFkB assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample.
Benefits
- SPECIFICITY
- PRECISION
- DATA NORMALIZATION
Total-NFkB assay principle
Total-NFkB 2-plate assay protocol
Total-NFkB 1-plate assay protocol
Detection of total NFkB in various human/mouse cells
HTRF assay vs WB using total-NFkB assay

TNFa dose-response on HeLa cells using phospho and total NFkB kit
Pharmacological response on Phospho and total NFkB of BAY 11-7085
Regulation of the NFkB pathway
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Notes d'application
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Présentations scientifiaues
Cisbio lysis buffer compatibility
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
physiologically relevant results fo fast flowing research - Flyers
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Notes d'application
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Notes d'application
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Notes d'application
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Notes d'application
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Notes techniques
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Notes d'application
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Notes techniques
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Notes techniques
How to run a cell based phospho HTRF assay
What to expect at the bench - Vidéos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Vidéos
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Présentations scientifiaues
STING HTRF offer to bridge innate and adaptive immunity
cGAS-STING signaling pathway from A to Z - Brochures
Product Insert NFkB total Kit / 64NFTPEG-64NFTPEH
64NFTPEG-64NFTPEH - Protocoles
All your HTRF assays in one document! - Catalogue
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Novel HTRF platform to delineate STING pathway
Explore the whole STING pathway with a single technology - Posters
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
STING, the next candidate for cancer immunotherapy
Infography about STING protein - Infographies
A Guide To Current Therapies in Immuno Oncology
Comprehensive White Paper about immuno-oncology - Guides
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Vidéos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Vidéos
Characterize STING agonists with HTRF human IFNb
Data about HTRF IFNb correlation with gene reporter assay and ELISA - Notes d'application
HTRF HMGB1, IL-1β and IL-18 assays discriminate between inflammasome triggered pyroptosis, and necroptosis - Notes d'application
Neuroinflammation study combining hiPSC-derived astrocytes and HTRF
See published experiments and data demonstrating how immunoassays rise to the challenge of astrocyte studies in neuroinflammation research - Notes d'application
On-demand webinar: Linking Neuroinflammation and Neurodegeneration
New insight into neuroinflammation research - Vidéos
Assays for autoimmunity research
Advance your research on autoimmune diseases - Flyers
Cell-based assays for neurosciences
HTRF solutions - Flyers
Advance your research on Fibrosis
Kits and reagents for Fibrosis research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Notes techniques
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Notes d'application
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Notes d'application
Safety Data Sheet NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (DEU) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (ELL) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (FRA-FR) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (ITA) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (SPA) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (ENG-GB) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet (ENG-US) NFkB total Kit / 64NFTPEG
64NFTPEG - Fiches de sécurité
Safety Data Sheet NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (DEU) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (ELL) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (FRA-FR) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (ITA) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (SPA) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (ENG-GB) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (ENG-US) NFkB total Kit / 64NFTPEH
64NFTPEH - Fiches de sécurité
Safety Data Sheet (DEU) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
Safety Data Sheet (ELL) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
Safety Data Sheet (FRA-FR) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
Safety Data Sheet (ITA) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
Safety Data Sheet (SPA) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
Safety Data Sheet (ENG-GB) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
Safety Data Sheet (ENG-US) NFkB total Kit / 64NFTPEY
64NFTPEY - Fiches de sécurité
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