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Phospho-AKT1/2/3 (Ser473) control lysate HTRF®

This lysate is a component of the phospho-AKT1/2/3 (Ser473) kit. It may be used as a residue specific positive control for AKT phosphorylation.
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This lysate is a component of the phospho-AKT1/2/3 (Ser473) kit. It may be used as a residue specific positive control for AKT phosphorylation.
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Overview

This cell lysate provides a way to validate the phospho-AKT1/2/3 (Ser473) assay. As a positive control, it will enable researchers to avoid any detection issues when running the assay. The lysate was made from stimulated CHO cells.

Benefits

  • ENSURE REAGENT INTEGRITY
  • FACILITATE EXPERIMENTAL TROUBLESHOOTING

Inflammation cell by cell

HTRF solutions for each cell type - Flyers

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalogue

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences

Insider Tips for successful sample treatment - Notes techniques

Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Notes techniques

Best practices for analyzing tumor xenografts with HTRF phospho assays

Protocol for tumor xenograft analysis with HTRF - Notes techniques

Key guidelines to successful cell signaling experiments

Mastering the art of cell signaling assays optimization - Guides

HTRF® cell signaling platform combined with iCell® Hepatocytes

A solution for phospho-protein analysis in metabolic disorders - Posters

HTRF phospho-assays reveal subtle drug-induced effects

Detailed protocol and direct comparison with WB - Posters

Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

Tumor xenograft analysis: HTRF versus Western blot - Notes d'application

HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Notes d'application

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Notes d'application

HTRF Alpha-tubulin Housekeeping kit

Properly interpret your compound effect - Notes d'application

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Notes d'application

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Vidéos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Vidéos

How to run a cell based phospho HTRF assay

What to expect at the bench - Vidéos

HTRF cellular phospho-protein assays

physiologically relevant results fo fast flowing research - Flyers

Signaling pathways in liquid tumors

This litterature review highlights the signaling pathways and give examples of how HTRF® KinEASE™ and AlphaLISA® SureFire® assays can be used in the development of novel therapies for leukemia treatment - Notes d'application

Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection

Seeding and lysing recommendations for a number of cell culture vessels. - Notes techniques

Safety Data Sheet AKT P-S473 Ctl / 62AKSTDA

62AKSTDA - Fiches de sécurité

Batch Information AKT P-S473 Ctl / 20220319

62AKSTDA Lot 11A - Informations techniques

Batch Information AKT P-S473 Ctl / 20220605

62AKSTDA Lot 12A - Informations techniques

Batch Information AKT P-S473 Ctl / 20220930

62AKSTDA Lot 13A - Informations techniques

Batch Information AKT P-S473 Ctl / 20230109

62AKSTDA Lot 14A - Informations techniques

Batch Information AKT P-S473 Ctl / 20231023

62AKSTDA Lot 15A - Informations techniques

Batch Information AKT P-S473 Ctl / 20240216

62AKSTDA Lot 16A - Informations techniques

Batch Information AKT P-S473 Ctl / 20240930

62AKSTDA Lot 17A - Informations techniques

Plate Reader Requirement

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