A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phospho AKT-Eu cryptate HTRF®
- High affinity
The Anti-phospho AKT-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of Akt only when phosphorylated at Ser473. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSPHO-AKT (SER473) SPECIFIC
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Notes d'application