cAMP Gs dynamic kit
Optimal performance when applied to Gs activation or inhibition
IP-One Gq kit HTRF®
This HTRF kit detects the accumulation of inositol monophosphate, a metabolite produced following phospholipase C activation and used as a proxy of Gq activation
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Ease-of-use
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Low false negative
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Highly specific
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Accurate pharmacology
Overview
Cisbio IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.
From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays
Benefits
- SPECIFICALLY TIED TO GQ SIGNALING
- LOW FALSE NEGATIVES
- ACCURATE PHARMACOLOGICAL PROFILES
Assay principle
The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.
Assay protocol
The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.
Specificity of IP-One Gq assay
IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs.
In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.
Standard curve characteristics
Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !
Experience on HTRF technology in public-private collaborations
Neogenius Pharma - Présentations scientifiaues
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Présentations scientifiaues
In collaboration with the Faculty of health and medical sciences Denmark - Présentations scientifiaues
In collaboration with the Faculty of health and medical sciences Denmark - Présentations scientifiaues
In collaboration with MRC technology - Présentations scientifiaues
HTRF a versatil approach for 7TM drug discovery
In collaboration with MRC - Présentations scientifiaues
In collaboration with Roche - Présentations scientifiaues
In collaboration with Novartis - Présentations scientifiaues
Case Study: HTS of GPR-54 with IP-One Assay
In collaboration with Harvard Center for Neurodegeneration and Repair - Présentations scientifiaues
cAMP Fresh and Frozen Cell Usage
In collaboration with Bristol Myers Squibb - Présentations scientifiaues
IP-One & cAMP HTRF Functional Cell-based Screening
In collaboration with Schering AG - Présentations scientifiaues
In collaboration with Novartis - Présentations scientifiaues
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF
In collaboration with College of Science, King Saud University, University of Western Australia - Posters
HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
In collaboration with Mollecular Devices - Posters
Multidimentional GPCR investigations - Posters
In collaboration with CEREP - Posters
Differentiation of partial and full GPR40 agonists for the treatment of type 2diabetes
In collaboration with Jansen research & development - Posters
In collaboration with Jansen research & development - Posters
Ultimate guide to master Gq assays
Optimizing IP-One assays has never been easier - Guides
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Guidelines and recommendations for analyzing GPCR 2nd messenger data
Written by a panel of experts - Guides
Measuring Gq activation on brain tissues - Posters
Unique receptor dissociation kinetics of the novel endothelin receptor antagonist macitentan
Characterizing a drug candidate for Gq coupled GPCR - Posters
How to monitor Gq coupled GPCR activation in cells with IP-One
What to expect at the bench - Vidéos
IP-One, a new dimension in Gq signaling
Video guide - Vidéos
HTRF IP-One assay: a robust and stable platform for high throughput screening of Gaq-coupled GPCRs
Overcome calcium assay signal's transient nature in Gq studies - Notes techniques
Immuno-Oncology Drug Discovery
Explore our solutions for Immuno-oncology research - Flyers
All your HTRF assays in one document! - Catalogue
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
GPCR signaling in cardiovascular research addressed with HTRF 2nd messenger assays
See how peer researchers monitor GPCR signaling with HTRF - Notes d'application
A comprehensive overview of pharmacology's main ligands - Guides
Fibrosis study investigating the role of GPCR signaling in fibrosis development - Notes d'application
GTP binding assay : Gαi agonist and antagonist guides
Discover how to get the most out of your Gαi agonist and antagonist research with our HTRF GTP binding assays. - Guides
GPCR compounds identification and pharmacological characterization with GTP Gi binding assay
Characterization of candidate molecules through GTP binding assays - Notes d'application
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Manual IP-One Gq / 62IPAPEB-62IPAPEC-62IPAPEJ
62IPAPEB-62IPAPEC-62IPAPEJ - Protocoles
Certificate of Analysis IP-One Gq / 62IPAPEB
62IPAPEB Lot 17C - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEB
62IPAPEB Lot 17D - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEB
62IPAPEB Lot 17E - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEB
62IPAPEB Lot 17F - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEB
62IPAPEB Lot 17G - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEB
62IPAPEB Lot 17H - Rapports de contrôle qualité
Safety Data Sheet (ELL) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Safety Data Sheet (FRA-FR) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Safety Data Sheet (ITA) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Safety Data Sheet (SPA) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Safety Data Sheet (DEU) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Safety Data Sheet (ENG-GB) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Safety Data Sheet (ENG-US) IP-One Gq / 62IPAPEB
62IPAPEB - Fiches de sécurité
Certificate of Analysis IP-One Gq / 62IPAPEC
62IPAPEC Lot 22E - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEC
62IPAPEC Lot 22F - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEC
62IPAPEC Lot 22G - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEC
62IPAPEC Lot 22H - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEC
62IPAPEC Lot 22J - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEC
62IPAPEC Lot 22K - Rapports de contrôle qualité
Safety Data Sheet (DEU) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Safety Data Sheet (ELL) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Safety Data Sheet (FRA-FR) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Safety Data Sheet (ITA) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Safety Data Sheet (SPA) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Safety Data Sheet (ENG-GB) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Safety Data Sheet (ENG-US) IP-One Gq / 62IPAPEC
62IPAPEC - Fiches de sécurité
Certificate of Analysis IP-One Gq / 62IPAPEJ
62IPAPEJ Lot 22D - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEJ
62IPAPEJ Lot 22E - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEJ
62IPAPEJ Lot 22F - Rapports de contrôle qualité
Certificate of Analysis IP-One Gq / 62IPAPEJ
62IPAPEJ Lot 22H - Rapports de contrôle qualité
Safety Data Sheet (DEU) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
Safety Data Sheet (ELL) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
Safety Data Sheet (FRA-FR) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
Safety Data Sheet (ITA) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
Safety Data Sheet (SPA) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
Safety Data Sheet (ENG-GB) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
Safety Data Sheet (ENG-US) IP-One Gq / 62IPAPEJ
62IPAPEJ - Fiches de sécurité
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