Human IFN gamma kit
No-wash kit to quantify released Human IFN gamma
The HTRF human IL2 kit is designed for the quantification of human IL2 release in cell supernatant.
IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes the differentiation of T cells into effector or memory T cells, thus helping the body fight off infection. IL2 is also a key player in immuno-oncology since it is the main promoter of the differentiation of T cells into Treg (regulatory T cells).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-2 AlphaLISA kitand the Human IL-2 AlphaLISA biotin-free kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL2 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||7 pg/mL &37 pg/mL|
|Range||37 8,000 pg/mL|
|Time to result||3h at RT|
|Calibration||NIBSC (86/500) value (IU/mL) = 0,01 x HTRF hIL2 value (pg/mL)|
|Sample||Mean [IL2] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[IL2] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Jurkat cells plated at 50 and 100 kcells/well were stimulated for 24 h with anti CD3 & and anti CD28 respectively prepared at 10 and 1 µg/mL. 16 µL of supernatants were transferred into a white detection plate (384 low volume) to be analyzed by the Human IL2 Assay.
In collaboration with CNRS - Présentations scientifiaues
In collaboration with Novartis - Présentations scientifiaues
In collaboration with Blood assay solution - Notes d'application
Perform and optimize cytokine assays on PBMC - Notes techniques
A fun video introducing you to cytokines assays with HTRF - Vidéos
cGAS-STING signaling pathway from A to Z - Brochures
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
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