Human CCL3 (MIP1 alpha) kit
No-wash kit to quantify released Human CCL3 (MIP1 alpha)
The HTRF human IL1 alpha kit is designed for the quantification of human IL1 alpha release in cell supernatant.
Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-1 alpha AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL1 alpha analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||8 pg/mL &20.5 pg/mL|
|Range||20.5 – 5,000 pg/mL|
|Time to result||2h at RT|
|Calibration||NIBSC (86/632) value (IU/mL) = 10 x HTRF hIL1a value (pg/mL)|
|Sample||Mean [IL1a] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV iwas calculated for each sample.
|Sample||[IL1a] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Présentations scientifiaues
In collaboration with Blood assay solution - Notes d'application
Perform and optimize cytokine assays on PBMC - Notes techniques
A fun video introducing you to cytokines assays with HTRF - Vidéos
Explore the whole STING pathway with a single technology - Posters
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
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