Human CXCL1 (GRO alpha) kit
No-wash kit to quantify released Human CXCL1 (GRO alpha)
The HTRF human CXCL10 (IP-10) kit is designed for the quantification of human CXCL10 release in cell supernatant.
Also called IP-10 (interferon gamma-induced protein 10) or small-inducible cytokine B10, CXCL10 is a chemokine secreted by monocytes, fibroblasts, and endothelial cells in response to IFN gamma. IP-10's main effect is to promote cell migration through Calcium signaling. Target cells include macrophages, T cells, NK cells, and dendritic cells. CXCL10 has significant anti-tumor effects, as well as anti-angiogenic effects.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human CXCL10/IP-10 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CXCL10 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||7 pg/mL & 26 pg/mL|
|Range||26 – 5,000 pg/mL|
|Time to result||2h at RT|
|Sample||Mean [CXCL10] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[CXCL10] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Présentations scientifiaues
In collaboration with Blood assay solution - Notes d'application
Perform and optimize cytokine assays on PBMC - Notes techniques
A fun video introducing you to cytokines assays with HTRF - Vidéos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
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