Human CCL5 (RANTES) kit HTRF®
The HTRF human CCL5 (RANTES) kit is designed for the quantification of human CCL5 release in cell supernatant.
- Low sample consumption
- Under 2h bench time
CCL5, also known as RANTES (regulated upon activation, normal T-cell expressed and secreted), is a chemokine mainly secreted by T lymphocytes, macrophages, platelets, and certain types of tumor cells. RANTES plays an important role in inflammatory sites by recruiting T cells and macrophages. When secreted with IL-2 and IFN-γ, RANTES also induces the activation and proliferation of natural killer (NK) cells. The function of RANTES in oncology is still unclear, but in infectious diseases RANTES has been described as inhibiting HIV entry into target cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human CCL5/RANTES AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
- NO CROSS WITH CCL3 OR 4
- CYTOTOXIC T CELLS READOUT
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL5 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol|
|LOD &LOQ (in Diluent)||7 pg/mL &18 pg/mL|
|Range||18 2,000 pg/mL|
|Time to result||ON @ RT|
|Calibration||NIBSC (92/520) value (IU/mL) = 0,015 x HTRF hCCL5 value (pg/mL)|
Intra assay (n=24)
|Sample||Mean [CCL5] (pg/mL)||CV|
Inter assay (n=4)
|Sample||[CCL5] (pg/mL)||Mean (delta ratio)||CV|
LX2 cells were plated at 400,000 cells/well in a 6 well plate, cultured for 3 days, then starved for 1 day before 48 hours of stimulation with TGFß at 0.5ng/ml. Next, 16 µL of supernatant were transferred into a 384 well (SV) white detection plate to be analyzed with the Human CCL5 Assay.
In collaboration with CNRS - Présentations scientifiaues
In collaboration with Blood assay solution - Notes d'application
Perform and optimize cytokine assays on PBMC - Notes techniques
A fun video introducing you to cytokines assays with HTRF - Vidéos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
Plate Reader Requirement
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