Histamine Dynamic kit
Histamine quantification from Blood, PBMCs and cells
The Histamine HTRF kit is a highly specific assay for quantifying an allergic response in whole blood and cell-based conditions.
Histamine (1H-imidazole 4-ethaneamine) is a biogenic amine known to play a key role in immediate hypersensitivity and inflammation reactions, as well as in several atopic diseases. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Bacterial decarboxylation of the amino acid histidine in fresh fish produces histamine, which can be used as a health hazard biomarker indicative of bacterial contamination. The histamine kit accurately measures histamine from PBMCs and cell-based formats.
The histamine assay features a streamlined protocol with only two incubation steps:
- Sample with acylation buffer and acylation reagent
- Histamine detection using HTRF reagents.
This protocol requires a single, three-hour incubation period following the acylation step.
|Standard curve||0.19 - 3,000 nM|
|Limit of detection (LoD)||0.21 nM|
|Assay range (LoD - IC90)||0.21 - 156 nM|
|Specificity:||Acylated histamine 100% |
Acylated L-histidine < 0.00067%
Acylated serotonin < 0.0067%
Four independent blood samples (Donor A to D) were used to measure the spontaneous release of histamine, as well as upon stimulation with 5 allergen mixes including 6 grass mix, house dust mite, cat epithelium, inhalant mix and common ragweed allergen.
*The anti IgE antibody (Ref # MABETS07) used as a positive control in the experiment can be purchased separately
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