Microplate readers

Performance, Versatility, and Reliability for Today’s Laboratory Needs

Essential companion to take your research further

All applications require specific detection conditions for an optimal readout. HTRF®, Alpha, LANCE® Ultra, or DELFIA®, all these high-performing technologies may differ in their detection modes and need a powerful and reliable instrument.

Choosing the suitable microplate reader ensures you’ll get an optimal readout. A multimodal reader gives your research the ideal flexibility and expands the field of possibilities by measuring a wide range of technologies.

Discover our high performance reader, or verify if your lab equipment is going to be compatible with our detection technologies.

Essential Microplate Reader

High Performance Readers

A powerful and reliable plate reader is what you need! Get access to readers which will ensure an optimal readout.

VICTOR® Nivo™

VICTOR® Nivo

Industry’s Smallest Multimode Plate Reader

VICTOR® Nivo packs all the latest major detection technologies into the industry’s smallest benchtop footprint. The perfect microplate reader for everyday biochemical and cell-based assays.

EnSight®

EnSight®

Industry-leading Technology Combination

EnSight® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. It is the perfect reader for high flexibility and fast results.

EnVision®

EnVision®

Our Fastest Benchtop Multimode Plate Reader

EnVision® provides ultra-high throughput and maximum sensitivity across all detection technologies. It is ideal for complex assays to drive your scientific breakthroughs.

Certified HTRF Readers and Setups

Already more than 50 certified or compatible instruments, and counting!

HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab.

You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup.

Check your reader’s compatibility and setup

Reader Control kit

The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. The kit includes everything you need: reagents, buffers and a microplate. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or different reader setup conditions.

Applications

  • Calibrate all HTRF-compatible readers
  • Validate readers following their upgrade to HTRF compatibility
  • Re-evaluate reader performance over time
  • Identify potential failure

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Interested in finding out more about the benefits of using HTRF Technology?

Learn more on HTRF technology

Many basic fluorescence plate readers are not equipped to run Alpha assays. To ensure the best possible results, we recommend using our VICTOR® Nivo, EnSight®, or EnVision® multimode plate reader, each of which comes with pre-set Alpha settings. You can use the same generic instrument protocol for AlphaScreen and AlphaLISA assays.

If you are using a plate reader from another company, we recommend that you check to see if the manufacturer has validated the instrument for an AlphaScreen or AlphaLISA assay. This information is usually found on the company’s website. PerkinElmer has not validated other instruments, and we would recommend that you speak to the instrument manufacturer for information on the recommended settings to use.

Instrument Settings

Already equipped with a PerkinElmer reader? You are all set! Our reader comes with pre-set Alpha settings ensuring you’ll get the most out of your assay with your reader.

Alpha technologies require excitation at 680 nm and reading emission at 520-620 nm (for AlphaScreen) or at 615 nm (for AlphaLISA). A laser excitation source is usually needed, as an intense light source is required to generate the signal for fast and sensitive readout. Xenon Flashlamp based systems and luminometers will not work for Alpha assays.

Interested in finding out more about the benefits of using Alpha Technology?

Learn more on AlphaLISA technology

LANCE Ultra assays require a microplate reader that is able to perform time-resolved fluorescence. We recommend filter-based fluorometers, though monochromator instruments can be used taking into account possibly decreased assay sensitivity. We recommend using our VICTOR® Nivo, EnSight®, or EnVision® multimode plate reader with the appropriate filters for these assays.

If you are using an instrument manufactured by another company, we recommend that you contact their technical support team to see if they have optimized settings, filter recommendations, etc. for a LANCE TR-FRET assay.

Instrument Settings

Use an excitation wavelength of 320 or 340 nm to excite the LANCE Europium chelate. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC, ULight dye, and Alexa Fluor 647). The raw FRET signal at 665 nm can be used to process your data. The ability to analyze the signal at 615 nm can be helpful when troubleshooting.

Interested in finding out more about the benefits of using LANCE Ultra Technology?

Learn more on LANCE Ultra technology

DELFIA assays require a plate reader that is capable of TRF (time-resolved fluorescence) detection. This is a time-resolved fluorescence intensity assay. We recommend using our VICTOR® Nivo, EnSight®, or EnVision® multimode plate reader.

We recommend a time-resolved filter-based fluorometer for DELFIA Europium assays. Very sensitive time-resolved spectrofluorometers may be suitable for certain assays. Most plate readers are capable of TRF-type assays – a flash lamp is needed for TRF analysis, which is an option on most plate readers. You could also use laser excitation on an appropriate EnVision instrument.

Instrument Settings

When using DELFIA Europium, we recommend generic default instrument settings for all DELFIA assays.

We always recommend that you check with the manufacturer of your particular instrument to see if they have additional recommendations for DELFIA assays.

Interested in finding out more about the benefits of using DELFIA Technology?

Learn more on DELFIA technology