IBMX Phosphodiesterase inhibitor
IBMX
cAMP Gs dynamic kit HTRF®
This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition
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Ease-of-use
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High sensitivity
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Accurate pharmacology
Overview
Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.
Cisbio's cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity
Benefits
- SCREENING OF GS COUPLED GPCR
- LEAD OPTIMIZATION OF GS COMPOUNDS
- STUDY OF GS GPCR RESPONSES
Assay principle
cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells. Native cAMP produced by cells competes with d2-labeled cAMP for binding to monoclonal anti-cAMP Eu3+ cryptate.
Assay protocol
The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents
cAMP Gs dynamic product specifications
Use this optimized kit to study your most challenging and precious cellular models.
Designed to produce results with minimal cell consumption, this kit features the lowest nanomolar IC50.
Left-shifted IC50 enables you to quantify lower cAMP concentrations, significantly reducing the number of cells required.
| S/B | IC20 nM | IC50 nM | IC80 nM | |
| cAMP - Gs dynamic | >14 | 0.91 | 4.07 | 18.1 |
| cAMP - Gs HiRange | >28 | 4.27 | 22.8 | 121 |
Validation on agonist dose response
HEK293 cells expressing the endogenous ß2 adrenergic receptor were treated with the isoproterenol agonist for 45min.
Isoproterenol was well characterized, displaying the right potency in good correlation with the literature.
Validation on antagonist dose response
HEK293 cells at 7,500 cells/well (optimal cellular concentration situated in the dynamic range of the kit) were treated with a serial concentration of isoproterenol, a beta 2 adrenergic receptor full-agonist.
The EC80-EC90 was determined to choose the optimal agonist concentration to use for the antagonist mode experiment.
Fitting the serial dilution of the antagonist allowed IC50 calculation for the compounds (potency) which was in perfect correlation with the literature.
In collaboration with CNRS - Présentations scientifiaues
In collaboration with the Faculty of health and medical sciences Denmark - Présentations scientifiaues
In collaboration with the Faculty of health and medical sciences Denmark - Présentations scientifiaues
In collaboration with MRC technology - Présentations scientifiaues
HTRF a versatil approach for 7TM drug discovery
In collaboration with MRC - Présentations scientifiaues
In collaboration with Novartis - Présentations scientifiaues
cAMP Fresh and Frozen Cell Usage
In collaboration with Bristol Myers Squibb - Présentations scientifiaues
IP-One & cAMP HTRF Functional Cell-based Screening
In collaboration with Schering AG - Présentations scientifiaues
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
Application of Bioassay Performance Monitoring Process with Statistical Tools
In collaboration with Amylin Pharmaceuticals Inc., Biocon Limited - Posters
In collaboration with Molecular Devices - Posters
Nanobodies and HTRF® technology to address an orphan class B GPCR: The GPR97 receptor
In collaboration with Sanofi - Posters
Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF
In collaboration with College of Science, King Saud University, University of Western Australia - Posters
HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
In collaboration with Mollecular Devices - Posters
Structure-activity characterization of glucagon receptor, an example of using HTRF®cAMP assay
In collaboration with Chinese Academy of Sciences - Posters
Multidimentional GPCR investigations - Posters
In collaboration with CEREP - Posters
Differentiation of partial and full GPR40 agonists for the treatment of type 2diabetes
In collaboration with Jansen research & development - Posters
Webinar: Advancing GPCR drug discovery through Allosteric Modulation
Featuring a panel of experts - Vidéos
Ultimate guides to master Gs and Gi GPCR assays
Optimizing cAMP assays has never been easier - Guides
Webinar: High-Throughput Screening of GPCRs for Drug Discovery Applications
Featuring a panel of experts - Vidéos
HTRF and Tag-lite technologies in multidimensional cell-based assays for GPCR investigations
4 GPCR challenges, 1 solution - Posters
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Guidelines and recommendations for analyzing GPCR 2nd messenger data
Written by a panel of experts - Guides
What to expect at the bench - Vidéos
Product Insert cAMP Gs dyn / 62AM4PEB-62AM4PEC-62AM4PEJ
62AM4PEB-62AM4PEC-62AM4PEJ - Protocoles
Quantitate cAMP and TNF Utilizing the Synergy 2 Multi-Detection Microplate Reader and HTRF assays
BioTek - Notes d'application
All your HTRF assays in one document! - Catalogue
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
GPCR signaling in cardiovascular research addressed with HTRF 2nd messenger assays
See how peer researchers monitor GPCR signaling with HTRF - Notes d'application
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographies
A comprehensive overview of pharmacology's main ligands - Guides
Three drug discovery approaches for Multiple Sclerosis
Power up your multiple sclerosis research projects with this technical note - Notes d'application
GPCR compounds identification and pharmacological characterization with GTP Gi binding assay
Characterization of candidate molecules through GTP binding assays - Notes d'application
Upstream/Downstream GPCR Readout Monitoring with GTP Gi Binding and cAMP Gi HTRF Assays
Read this note featuring two case studies that demonstrate the relevance of combining upstream (GTP) and downstream (cAMP) readouts for characterizing pharmacological compounds - Notes d'application
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Cyclic-AMP: A Functional Second Messenger for Assaying GPCR Target Activation
Selection of kits to detect of a broad range of cAMP - Notes d'application
Safety Data Sheet cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (DEU) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (ELL) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (FRA-FR) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (ITA) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (SPA) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (ENG-GB) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet (ENG-US) cAMP Gs dyn / 62AM4PEB
62AM4PEB - Fiches de sécurité
Safety Data Sheet cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (DEU) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (ELL) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (FRA-FR) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (ITA) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (SPA) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (ENG-GB) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Safety Data Sheet (ENG-US) cAMP Gs dyn / 62AM4PEC
62AM4PEC - Fiches de sécurité
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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