Angiotensin AT2R expressing cells labeled with Terbium
Tag-lite cells expressing the Angiotensin AT2 receptor with Terbium
Angiotensin AT2R fluorescent probe
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
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Non-radioactive
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No-wash
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Cell-based
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Equilibrium & Kinetic binding compatible
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Angiotensin AT2 receptor binding assay.
Benefits
- FLUORESCENTLY LABELED LIGAND
- HIGHLY STABLE
- VALIDATED IN BINDING
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Présentations scientifiaues
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Présentations scientifiaues
Tag-lite binding assays
The easy way to approach biology without radioactivity - Flyers
Determination of association (kon) and dissociation (koff) rates constants using the Tag-lite platform
Challenge the limits of binding kinetics studies - Notes d'application
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Webinar: The kinetics of drug-receptor binding: why it is important and how we can measure it
Featuring a panel of experts - Vidéos
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Vidéos
Product Insert Angioten-R red ago / L0007RED
L0007RED - Protocoles
Cisbio Product Catalog 2019
All your HTRF assays in one document! - Catalogue
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Vidéos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Notes d'application
Safety Data Sheet Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (DEU) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (ELL) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (FRA-FR) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (ITA) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (SPA) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (ENG-GB) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité
Safety Data Sheet (ENG-US) Angioten-R red ago / L0007RED
L0007RED - Fiches de sécurité