Beta2 adrenergicR fluorescent probe
Tag-lite Beta2 adrenergic receptor fluorescent probe
Adrenergic beta1R expressing cells labeled with Terbium
Tag-lite cells transiently expressing the Adrenergic beta1 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
Voir plus d’informations
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Fully functional cell line
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Transient expression
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Ready for binding
Overview
Endothelin receptors can be divided into ETA and ETB receptors also called ETA1 and ETA2. Both receptors are involved in the treatment of hypertension.
Cells expressing the BETA1 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Benefits
- PRE-LABELED WITH TERBIUM
- FROZEN CELLS, READY TO BE USED
- CELLS/LIGAND VALIDATED FOR BINDING
Assay principle
Running your BETA1 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Kd and Ki validation
Examples of data obtained using Adrenergic beta1 labeled cells and their matching fluorescent ligand (L0023GRE). CGP-12177 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Présentations scientifiaues
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Présentations scientifiaues
Tag-lite binding assays
The easy way to approach biology without radioactivity - Flyers
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Challenge the limits of binding kinetics studies - Notes d'application
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In collaboration with GEN - Guides
Webinar: The kinetics of drug-receptor binding: why it is important and how we can measure it
Featuring a panel of experts - Vidéos
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Vidéos
Kinetics of the ß2 Adrenergic receptor binding and signaling
A look at the kinetics of cAMP production in cells - Notes d'application
Cisbio Product Catalog 2019
All your HTRF assays in one document! - Catalogue
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Vidéos
Product Insert Ab1 LabCell / C1TT1BETA1
C1TT1BETA1 - Protocoles
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Notes d'application
Safety Data Sheet Ab1 LabCell / C1TT1BETA1
C1TT1BETA1 - Fiches de sécurité