SNAP tag A2B AdenosineR cloned plasmid
Tag-lite plasmid cloned with a SNAP tag A2B Adenosine receptor
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Adenosine A2B receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Adenosine A2B receptor binding assay.
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells (C1TT1A2B) into each well, followed by 5 µL of labeled ligand (L0068RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Notes d'application